Guanidinium is a planar trigonal cation which is similar in size to the gramicidin channel pore. We measured the effect of guanidinium on the conductance properties of the gramicidin channel and theoretically evaluated its interactions with the β-6.3 channel interior using an energy minimization and conformational search approach. Guanidinium current (measured in the absence of other permeable ions) could not be detected directly (g(Guan)/g(K)
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